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Agent skills

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Agent skill

Flow Cytometry Pipeline

End-to-end flow, spectral, and mass cytometry (CyTOF) pipeline from raw FCS files to differentially abundant/expressed cell populations. Orchestrates the read -> compensate/unmix -> transform -> QC -> doublet-removal -> cluster-or-gate -> annotate -> diffcyt DA/DS chain with flowCore/CATALYST/dif...

ClaudeCodexOpenClawData & Analytics
GPTomics1,232
Agent skill

FCS File Handling

Reads, inspects, and writes Flow Cytometry Standard (FCS) files from conventional, spectral, and mass cytometry (CyTOF), and parses FlowJo/Cytobank/Diva workspaces. Covers FCS 2.0/3.0/3.1/3.2 internals ($PnE linear-vs-log, $DATATYPE, $SPILLOVER vs SPILL vs $COMP, $TIMESTEP), channel/parameter met...

ClaudeCodexOpenClawData & Analytics
GPTomics1,232
Agent skill

Compensation and Transformation

Spillover compensation and data transformation for flow cytometry. Covers compensation matrix calculation, application, and biexponential/arcsinh transforms. Use when correcting spectral overlap between fluorophores or transforming data for analysis.

ClaudeCodexOpenClawData & Analytics
David-Li04063
Apache-2.0View resource
Agent skill

FCS File Handling

Read and manipulate Flow Cytometry Standard (FCS) files. Covers loading data, accessing parameters, and basic data exploration. Use when loading and inspecting flow or mass cytometry data before preprocessing.

ClaudeCodexOpenClawData & Analytics
David-Li04063
Apache-2.0View resource
Agent skill

Gating Analysis

Manual and automated gating for defining cell populations in flow cytometry. Covers rectangular, polygon, and data-driven gates. Use when identifying cell populations through hierarchical gating strategies.

ClaudeCodexOpenClawData & Analytics
David-Li04063
Apache-2.0View resource
Agent skill

Doublet Detection

Detect and remove doublets from flow and mass cytometry data. Covers FSC/SSC gating and computational doublet detection methods. Use when filtering out cell aggregates before clustering or quantitative analysis.

ClaudeCodexOpenClawData & Analytics
David-Li04063
Apache-2.0View resource
Agent skill

Compensation and Transformation

Corrects fluorophore spillover (conventional compensation) or spectral overlap (spectral unmixing) and applies variance-stabilizing transforms (logicle/biexponential, arcsinh, log) for flow and mass cytometry. Covers spillover-matrix estimation from single-stain controls, AutoSpill, the spillover...

ClaudeCodexOpenClawEducation & Research
GPTomics1,232
Agent skill

Gating Analysis

Defines cell populations in flow and spectral cytometry through manual gates (rectangle, polygon, quadrant, boolean) and reproducible automated gating (openCyto gating templates, flowDensity data-driven thresholds, flowClust model-based gates), organized as a hierarchical GatingSet (flowWorkspace...

ClaudeCodexOpenClawEducation & Research
GPTomics1,232
Agent skill · Reference

bio-workflows-cytometry-pipeline

Agent skill for Researcher, Data Scientist, Business Intelligence Analyst, published by GPTomics. Project details and file access are provided by the publisher.

OpenClawEducation & Research
GPTomics1,232
Agent skill · Reference

COPYRIGHT NOTICE

Agent skill for Researcher, Clinical Research, Data Scientist, published by Rudson-Oliveira. Project details and file access are provided by the publisher.

Education & Research
Rudson-Oliveira0
Not specifiedView resource
Agent skill · Reference

bio-flow-cytometry-bead-normalization

Agent skill for Researcher, Data Scientist, Business Intelligence Analyst, published by Rudson-Oliveira. Project details and file access are provided by the publisher.

Education & Research
Rudson-Oliveira0
Not specifiedView resource

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